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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Polyglutamine-binding protein 1,PQBP1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Polyglutamine- binding protein 1, PQBP1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PQBP1,MRX2; MRX55; MRXS3; MRXS8; NPW38; RENS1; SHS; 38 kDa nuclear protein containing a WW domain; mental retardation, X-linked 2 (non-dysmorphic); nuclear protein containing WW domain 38 kD; polyglutamine tract-binding protein 1; polyglutamine-binding protein 1; polyglutamine binding protein 1
Search name
Bovine PQBP1 ELISA KIT ,Bovine MRX2 ELISA KIT ,Bovine MRX55 ELISA KIT ,Bovine MRXS3 ELISA KIT ,Bovine MRXS8 ELISA KIT ,Bovine NPW38 ELISA KIT ,Bovine RENS1 ELISA KIT ,Bovine SHS ELISA KIT ,Bovine 38 kDa nuclear protein containing a WW domain ELISA KIT ,Bovine mental retardation X-linked 2 ELISA KIT ,Bovine nuclear protein containing WW domain 38 kD ELISA KIT ,Bovine polyglutamine tract-binding protein 1 ELISA KIT ,Bovine polyglutamine-binding protein 1 ELISA KIT ,Bovine polyglutamine binding protein 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Polyglutamine- binding protein 1, PQBP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polyglutamine- binding protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Polyglutamine- binding protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Polyglutamine- binding protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Polyglutamine- binding protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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