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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Pyridoxine-5'-phosphate oxidase,PNPO
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Pyridoxine- 5'- phosphate oxidase, PNPO ELISA KIT
Product Name:Bovine Pyridoxine- 5'- phosphate oxidase, PNPO ELISA KIT
Packing:96T
Catalog No.:ELI-45244b
Gene Name:Bovine Pyridoxine-5'-phosphate oxidase, PNPO
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Pyridoxine-5'-phosphate oxidase, PNPO
Alternative Name:PNPO,Bovine Pyridoxine-5'-phosphate oxidase, PNPO
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Pyridoxine- 5'- phosphate oxidase, PNPO ELISA KIT allows for the in vitro quantitative determination of Bovine Pyridoxine-5'-phosphate oxidase, PNPO concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Pyridoxine- 5'- phosphate oxidase, PNPO ELISA KIT has been pre-coated with an Bovine Pyridoxine-5'-phosphate oxidase, PNPO antibody specific to Bovine Pyridoxine-5'-phosphate oxidase, PNPO .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Pyridoxine-5'-phosphate oxidase, PNPO and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Pyridoxine-5'-phosphate oxidase, PNPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Pyridoxine-5'-phosphate oxidase, PNPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
Methods for the Detection of D-Amino-Acid Oxidase
al. ( 5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
Procedures for In Vitro Production of Bovine Embryos
tubes (05-539-5)18 g 1 ½” needles (14-826-5D)50 ml sterile centrifuge tubes (05-538-67) 60 X 15 petri dishes (08757100B) (Falcon 351007) Absorbent paper (14-206-65)Beakers 10 ml (02-540C) 50 ml (02-540G) 400 ml (02-555
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