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Bovine Diacylglycerol O-acyltr

ansferase 1,DGAT1 ELISA KIT
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  • ¥3480
  • LSM Bio
  • 0.78-50ng/ml
  • 0.469ng/ml
  • 进口
  • ELI-46779b
  • 2025年07月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      100

    • 供应商

      LSM BIO

    • 检测范围

      0.78-50ng/ml

    • 检测方法

      夹心法

    • 应用

      利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度

    • 适应物种

    • 标记物

      Bovine Diacylglycerol O-acyltransferase 1,DGAT1

    • 样本

      牛的血浆,血清,细胞上清等

    • 灵敏度

      0.469ng/ml

    • 规格

      96tests

    Bovine Diacylglycerol O- acyltransferase 1, DGAT1 ELISA KIT

    Product Name:Bovine Diacylglycerol O- acyltransferase 1, DGAT1 ELISA KIT
    Packing:96T

    Catalog No.:ELI-46779b

    Gene Name:Bovine Diacylglycerol O-acyltransferase 1, DGAT1

    Detect Range:31.2-2000pg/ml

    Sensitivity:18.75pg/ml

    Target Protein Name:Bovine Diacylglycerol O-acyltransferase 1, DGAT1

    Alternative Name:DGAT1,Bovine Diacylglycerol O-acyltransferase 1, DGAT1
    Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    ELISA type:Sandwich ELISA Kit
    Product Description:Bovine Diacylglycerol O- acyltransferase 1, DGAT1 ELISA KIT allows for the in vitro quantitative determination of Bovine Diacylglycerol O-acyltransferase 1, DGAT1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    ELISA Test Principle:
    The microtiter plate provided in Bovine Diacylglycerol O- acyltransferase 1, DGAT1 ELISA KIT has been pre-coated with an Bovine Diacylglycerol O-acyltransferase 1, DGAT1 antibody specific to Bovine Diacylglycerol O-acyltransferase 1, DGAT1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Diacylglycerol O-acyltransferase 1, DGAT1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Diacylglycerol O-acyltransferase 1, DGAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Diacylglycerol O-acyltransferase 1, DGAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

     

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    图标文献和实验
    该产品被引用文献
    Article: Changes in nitric oxide, carbon monoxide, hydrogen sulfide and male reproductive hormones in response to chronic restraint stress in rats Author: AmiraMoustafa Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt Received 30 July 2020, Revised 1 September 2020, Accepted 22 October 2020, Available online 29 October 2020. Free Radical Biology and Medicine Available online 29 October 2020
    相关实验
    • 颜宁​团队 ​2021 首秀+ 2020 年度文章赏析,看大牛如何玩转结构生物学​!

      的偏好,并提供对 MBOAT 家族中酶催化机制的见解。 图片来自:Nature 3. 揭示人源二酰甘油酰基转移酶 (DGAT1) 三维结构及催化机制同期,2020 年 5 月 13 日,颜宁及周鸣团队在 Nature 在线发表题为 Structure and mechanism of human diacylglycerol O-acyltransferase 1 的研究论文,该工作利用单颗粒冷冻电镜技术, 首次解析了分辨率为 3.1Å 人源 DGAT1 的同源二聚体结构,并结合酶活性实验,确定

    • Procedures for In Vitro Production of Bovine Embryos

      Procedures for In Vitro Production of Bovine EmbryosThe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed

    • Sandwich ELISA Protocol

      4 2.0 g KCl Add ddH2O up to 10 L, pH to 7.2 with HCl PBS/Tween: 0.5 ml of Tween-20 in 1 L PBS Blocking Solution: 10% fetal bovine serum or 1% BSA in PBS. Filter before use to remove particulates

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    文献支持
    Bovine Diacylglycerol O-acyltransferase 1,DGAT1 ELISA KIT
    ¥3480