- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-46779b
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Diacylglycerol O-acyltransferase 1,DGAT1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Diacylglycerol O- acyltransferase 1, DGAT1 ELISA KIT
Product Name:Bovine Diacylglycerol O- acyltransferase 1, DGAT1 ELISA KIT
Packing:96T
Catalog No.:ELI-46779b
Gene Name:Bovine Diacylglycerol O-acyltransferase 1, DGAT1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Diacylglycerol O-acyltransferase 1, DGAT1
Alternative Name:DGAT1,Bovine Diacylglycerol O-acyltransferase 1, DGAT1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Diacylglycerol O- acyltransferase 1, DGAT1 ELISA KIT allows for the in vitro quantitative determination of Bovine Diacylglycerol O-acyltransferase 1, DGAT1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Diacylglycerol O- acyltransferase 1, DGAT1 ELISA KIT has been pre-coated with an Bovine Diacylglycerol O-acyltransferase 1, DGAT1 antibody specific to Bovine Diacylglycerol O-acyltransferase 1, DGAT1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Diacylglycerol O-acyltransferase 1, DGAT1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Diacylglycerol O-acyltransferase 1, DGAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Diacylglycerol O-acyltransferase 1, DGAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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4 2.0 g KCl Add ddH2O up to 10 L, pH to 7.2 with HCl PBS/Tween: 0.5 ml of Tween-20 in 1 L PBS Blocking Solution: 10% fetal bovine serum or 1% BSA in PBS. Filter before use to remove particulates
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