- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-46538b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Serine/threonine-protein kinase PAK 1,PAK1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Serine/threonine- protein kinase PAK 1, PAK1 ELISA KIT
Product Name:Bovine Serine/threonine- protein kinase PAK 1, PAK1 ELISA KIT
Packing:96T
Catalog No.:ELI-46538b
Gene Name:Bovine Serine(threonine-protein kinase PAK 1, PAK1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Serine(threonine-protein kinase PAK 1, PAK1
Alternative Name:PAK1,Bovine Serine(threonine-protein kinase PAK 1, PAK1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Serine/threonine- protein kinase PAK 1, PAK1 ELISA KIT allows for the in vitro quantitative determination of Bovine Serine(threonine-protein kinase PAK 1, PAK1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Serine/threonine- protein kinase PAK 1, PAK1 ELISA KIT has been pre-coated with an Bovine Serine(threonine-protein kinase PAK 1, PAK1 antibody specific to Bovine Serine(threonine-protein kinase PAK 1, PAK1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Serine(threonine-protein kinase PAK 1, PAK1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Serine(threonine-protein kinase PAK 1, PAK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Serine(threonine-protein kinase PAK 1, PAK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
.C., Asara, J.M., Lane, W.S., Garner, C.C., and Lienhard, G.E. 2002. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase‐activating protein (GAP) domain. J. Biol. Chem. 277:22115‐22118
. 1. Introduction Glycogen synthase kinase (GSK)-3 is a serine/threonine proteinkinase which was first described as a component of insulin-mediatedsignaling pathway (1) . There are two homologous mammalianisoforms, encoded by different genes, GSK-3a and GSK
(GST)或者是 GST fusion protein包含了Cdc42/Rac binding domain (CRIB) of PAK1 immobilized on glutathione–Sepharose 4B beads. The beads 用 lysis buffer清洗三次, bound GTP-Rac1 采用Rac1抗体进行免疫印渍分析 就我目前的了解来说,Rac和Cdc42都是可以采取这样的方式来检测其活性的.
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