- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-46147b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine LETM1 domain-containing protein 1,LETMD1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine LETM1 domain-containing protein 1, LTMD1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
LETMD1,110019O13Rik; HCCR-1; HCCR-2; HCCR1; HCRR-2; LETM1 domain-containing protein 1; cervical cancer 1 proto-oncogene protein p40; cervical cancer proto-oncogene 2 protein; regulator of TP53; LETM1 domain containing 1
Search name
Bovine LETMD1 ELISA KIT ,Bovine 110019O13Rik ELISA KIT ,Bovine HCCR-1 ELISA KIT ,Bovine HCCR-2 ELISA KIT ,Bovine HCCR1 ELISA KIT ,Bovine HCRR-2 ELISA KIT ,Bovine LETM1 domain-containing protein 1 ELISA KIT ,Bovine cervical cancer 1 proto-oncogene protein p40 ELISA KIT ,Bovine cervical cancer proto-oncogene 2 protein ELISA KIT ,Bovine regulator of TP53 ELISA KIT ,Bovine LETM1 domain containing 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine letm1 domain-containing protein 1,LTMD1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to LTMD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LTMD1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LTMD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTMD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Elite ABC kit) in 1 mL of PBS containing 0.1% BSA for 10 min at room temperature. ix. After incubation with secondary antibodies, rinse slides twice in PBS containing 0.1% BSA (10 min each). Remove excess liquid
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
tube containing 100 µl sample3.If desirred,add an equal volume of 1 M NaOH to each sample and vortex (see Comments below).Add NaOH to standards as well if this option is used.4.Prepare standards containing a range of 5 to 100 micrograms protein (albumin
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