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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Nuclear pore complex protein Nup93,NUP93
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Nuclear pore complex protein Nup93, NUP93 ELISA KIT
Product Name:Bovine Nuclear pore complex protein Nup93, NUP93 ELISA KIT
Packing:96T
Catalog No.:ELI-44229b
Gene Name:Bovine Nuclear pore complex protein Nup93, NUP93
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Nuclear pore complex protein Nup93, NUP93
Alternative Name:NUP93,Bovine Nuclear pore complex protein Nup93, NUP93
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Nuclear pore complex protein Nup93, NUP93 ELISA KIT allows for the in vitro quantitative determination of Bovine Nuclear pore complex protein Nup93, NUP93 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Nuclear pore complex protein Nup93, NUP93 ELISA KIT has been pre-coated with an Bovine Nuclear pore complex protein Nup93, NUP93 antibody specific to Bovine Nuclear pore complex protein Nup93, NUP93 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Nuclear pore complex protein Nup93, NUP93 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Nuclear pore complex protein Nup93, NUP93, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Nuclear pore complex protein Nup93, NUP93 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验施一公团队突破性进展再登 Science!以超高分辨率解析核孔复合物的结构细节
质组装成正确的三维分子结构一直是一个巨大的挑战。 2022 年 6 月 10 日,西湖大学施一公团队在 Science 杂志发表研究论文 Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex。 该研究聚焦于非洲爪蟾核孔复合物(NPC)结构,首次扩展了脊椎动物细胞质环(CR)亚单位复合体模型的分子量,获得高分辨率的单粒子冷冻电镜显微结构。除了解析 Y 复合物,同时发现 5 个核孔蛋白 Nup358
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Identification of Protein Interactions by Far Western Analysis
protein of interest cloned into an in vitro expression vector In vitro transcription/translation kit (Promega) 10 mCi/ml 35
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