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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine N-acetylneuraminate lyase,NPL
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine N- acetylneuraminate lyase, NPL ELISA KIT
Product Name:Bovine N- acetylneuraminate lyase, NPL ELISA KIT
Packing:96T
Catalog No.:ELI-44135b
Gene Name:Bovine N-acetylneuraminate lyase, NPL
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine N-acetylneuraminate lyase, NPL
Alternative Name:NPL,Bovine N-acetylneuraminate lyase, NPL
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine N- acetylneuraminate lyase, NPL ELISA KIT allows for the in vitro quantitative determination of Bovine N-acetylneuraminate lyase, NPL concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine N- acetylneuraminate lyase, NPL ELISA KIT has been pre-coated with an Bovine N-acetylneuraminate lyase, NPL antibody specific to Bovine N-acetylneuraminate lyase, NPL .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine N-acetylneuraminate lyase, NPL and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine N-acetylneuraminate lyase, NPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine N-acetylneuraminate lyase, NPL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【信息】Northstar BioProducts(NSBP)
This kit contains all enzymes needed to completely remove all N- & simple O-linked carbohydrates from glycoproteins 100471-1 Endo F Sampler Kit 1 kit Three recombinant endoglycosidases from Elizabethkingia meninoseptium – Endo F1
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
-MF[80]Candida rugosa LIP2 lipaseS, 2.3 mg/L, α-MF, active463Candida rugosa lip4 lipaseS, 100mg/L, α-MF, active459Fusarium solani pectate lyase (pelC)S, 1 mg/L, PHO1[81]Fusarium solani pectate lyase (pelD)S, native[82]Geotrichum candidum lipase
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
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