- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-43966b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Heat shock factor-binding protein 1,HSBP1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Heat shock factor- binding protein 1, HSBP1 ELISA KIT
Product Name:Bovine Heat shock factor- binding protein 1, HSBP1 ELISA KIT
Packing:96T
Catalog No.:ELI-43966b
Gene Name:Bovine Heat shock factor-binding protein 1, HSBP1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Heat shock factor-binding protein 1, HSBP1
Alternative Name:HSBP1,Bovine Heat shock factor-binding protein 1, HSBP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Heat shock factor- binding protein 1, HSBP1 ELISA KIT allows for the in vitro quantitative determination of Bovine Heat shock factor-binding protein 1, HSBP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Heat shock factor- binding protein 1, HSBP1 ELISA KIT has been pre-coated with an Bovine Heat shock factor-binding protein 1, HSBP1 antibody specific to Bovine Heat shock factor-binding protein 1, HSBP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Heat shock factor-binding protein 1, HSBP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Heat shock factor-binding protein 1, HSBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Heat shock factor-binding protein 1, HSBP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of Heat-Shock Transcription Factor and Element-Binding Activity
region of genes encoding heat-shock proteins (3 ). Heat-shock protein gene promoter contains three heat-shock elements (HSE, 5′-GAAnnTTCnnGAA-3′) (4 ). The HSE is known as a conserved motif present in the promoters of many heat-inducible heat-shock
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
for mapping distributions of chromosome-associated proteins, identifying and characterizing cis -regulatory DNA elements bound by particular proteins, and mapping functional protein domains necessary for chromosomal binding or other activities
maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs.Both hydrophobic and ionic interactions stabilize the anionic form of the dye,causing a visible color change.The assay is useful
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