- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-42703b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Histamine N-methyltransferase,HNMT
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Histamine N- methyltransferase, HNMT ELISA KIT
Product Name:Bovine Histamine N- methyltransferase, HNMT ELISA KIT
Packing:96T
Catalog No.:ELI-42703b
Gene Name:Bovine Histamine N-methyltransferase, HNMT
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Histamine N-methyltransferase, HNMT
Alternative Name:HNMT,Bovine Histamine N-methyltransferase, HNMT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Histamine N- methyltransferase, HNMT ELISA KIT allows for the in vitro quantitative determination of Bovine Histamine N-methyltransferase, HNMT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Histamine N- methyltransferase, HNMT ELISA KIT has been pre-coated with an Bovine Histamine N-methyltransferase, HNMT antibody specific to Bovine Histamine N-methyltransferase, HNMT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Histamine N-methyltransferase, HNMT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Histamine N-methyltransferase, HNMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Histamine N-methyltransferase, HNMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Histamine N-Methyltransferase: Enzymology and Functional Aspects
Histamine N -methyltransferase (HNMT; EC 2.1.1.8) was discovered a little over 25 yr ago. Since that time, HNMT has been the subject of numerous investigations, and although these studies have provided a fundamental understanding of this enzyme
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. A protein microarray ELISA for screening biological fluids. Methods Mol. Biol. 264: 161‐172. White, A.M., Daly, D.S., Varnum, S.M., Anderson, K.K., Bollinger, N., and Zangar, R.C
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