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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine LRRN4 C-terminal-like protein,LRRN4CL
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine LRRN4 C- terminal- like protein, LRRN4CL ELISA KIT
Product Name:Bovine LRRN4 C- terminal- like protein, LRRN4CL ELISA KIT
Packing:96T
Catalog No.:ELI-42217b
Gene Name:Bovine LRRN4 C-terminal-like protein, LRRN4CL
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine LRRN4 C-terminal-like protein, LRRN4CL
Alternative Name:LRRN4CL,Bovine LRRN4 C-terminal-like protein, LRRN4CL
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine LRRN4 C- terminal- like protein, LRRN4CL ELISA KIT allows for the in vitro quantitative determination of Bovine LRRN4 C-terminal-like protein, LRRN4CL concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine LRRN4 C- terminal- like protein, LRRN4CL ELISA KIT has been pre-coated with an Bovine LRRN4 C-terminal-like protein, LRRN4CL antibody specific to Bovine LRRN4 C-terminal-like protein, LRRN4CL .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine LRRN4 C-terminal-like protein, LRRN4CL and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine LRRN4 C-terminal-like protein, LRRN4CL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine LRRN4 C-terminal-like protein, LRRN4CL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Identification of Protein Interactions by Far Western Analysis
protein of interest cloned into an in vitro expression vector In vitro transcription/translation kit (Promega) 10 mCi/ml 35
. Fig. 1 Glycerol gradient sedimentation analysis of SMC protein from B. subtilis (BsSMC ; upper panel ) and sedimentation standards catalase and bovine serum albumin (lower panel ). A 200-μl sample was layered on a 5.0-ml gradient of 15�40
Antigen Design Sera Purification Tech Sheet
and may have some degree of secondary structure. Coupling Strategy A factor that is often over-looked when designing a synthetic peptide is the method of coupling the peptide to the carrier protein. For example, N-terminal sequences should be coupled through the C
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