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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Catechol O-methyltransferase,COMT
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Catechol O- methyltransferase, COMT ELISA KIT
Product Name:Bovine Catechol O- methyltransferase, COMT ELISA KIT
Packing:96T
Catalog No.:ELI-32353b
Gene Name:Bovine Catechol O-methyltransferase, COMT
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine Catechol O-methyltransferase, COMT
Alternative Name:COMT,Bovine Catechol O-methyltransferase, COMT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Catechol O- methyltransferase, COMT ELISA KIT allows for the in vitro quantitative determination of Bovine Catechol O-methyltransferase, COMT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Catechol O- methyltransferase, COMT ELISA KIT has been pre-coated with an Bovine Catechol O-methyltransferase, COMT antibody specific to Bovine Catechol O-methyltransferase, COMT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Catechol O-methyltransferase, COMT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Catechol O-methyltransferase, COMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Catechol O-methyltransferase, COMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Catechol-O -methyltransferase (COMT) is an enzyme that plays a key role in the modulation of catechol-dependent functions such as cognition, cardiovascular function, and pain processing. Recently, our group demonstrated
8.8 40mM MgCl2 160 mM NH4 (SO4)2 100 mM b -Mercaptoethanol 1 mg/ml bovine serum albumin. Tris-EDTA (TE) pH 8.0 DNA precipitation reagents: Phenol/Chloroform pH 8-9 3M NaOAc (for general precipitation) 5M NH4 Oac
内的肾上腺素与去甲肾上腺素通过单胺氧化酶(monoamine oxidase,MAO)与儿茶酚-O-甲基移位酶(catechol-O-methyltransferase,COMT)的作用而灭活。 (二)髓质激素的生物学作用 髓质与交感神经系统组成交感-肾上腺髓质系统,或称交感-肾上腺系统,所以,髓质激素的作用与交感神经紧密联系,难以分开。生理学家Cannon最早全面研究了交感-肾上腺髓质系统的作用,曾提出应急学说(emergency reaction hypothesis),认为机体
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