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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Serine/threonine-protein kinase 25,STK25
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Serine/threonine- protein kinase 25, STK25 ELISA KIT
Product Name:Bovine Serine/threonine- protein kinase 25, STK25 ELISA KIT
Packing:96T
Catalog No.:ELI-41335b
Gene Name:Bovine Serine(threonine-protein kinase 25, STK25
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Serine(threonine-protein kinase 25, STK25
Alternative Name:STK25,Bovine Serine(threonine-protein kinase 25, STK25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Serine/threonine- protein kinase 25, STK25 ELISA KIT allows for the in vitro quantitative determination of Bovine Serine(threonine-protein kinase 25, STK25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Serine/threonine- protein kinase 25, STK25 ELISA KIT has been pre-coated with an Bovine Serine(threonine-protein kinase 25, STK25 antibody specific to Bovine Serine(threonine-protein kinase 25, STK25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Serine(threonine-protein kinase 25, STK25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Serine(threonine-protein kinase 25, STK25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Serine(threonine-protein kinase 25, STK25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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family of serine/threonine-directed kinases that contain C-terminal kinase domains bearing significant sequence homology to those of mammalian and yeast phosphoinositide 3-kinases (1 ). In response to genotoxic insults, these kinases phosphorylate
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
.C., Asara, J.M., Lane, W.S., Garner, C.C., and Lienhard, G.E. 2002. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase‐activating protein (GAP) domain. J. Biol. Chem. 277:22115‐22118
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