- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-41118b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Rho GTPase-activating protein 15,ARHGAP15
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT
Product Name:Bovine Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT
Packing:96T
Catalog No.:ELI-41118b
Gene Name:Bovine Rho GTPase-activating protein 15, ARHGAP15
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine Rho GTPase-activating protein 15, ARHGAP15
Alternative Name:ARHGAP15,Bovine Rho GTPase-activating protein 15, ARHGAP15
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT allows for the in vitro quantitative determination of Bovine Rho GTPase-activating protein 15, ARHGAP15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT has been pre-coated with an Bovine Rho GTPase-activating protein 15, ARHGAP15 antibody specific to Bovine Rho GTPase-activating protein 15, ARHGAP15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Rho GTPase-activating protein 15, ARHGAP15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Rho GTPase-activating protein 15, ARHGAP15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Rho GTPase-activating protein 15, ARHGAP15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
Berman, D.M., Wilkie, T.M., and Gilman, A.G. 1996. GAIP and RGS4 are GTPase‐activating proteins for the Gi subfamily of G protein alpha subunits. Cell 86:445‐452.
) A proliferative role for Wnt-3ain chick somites. Dev Biol , 269 , 489-504. 10. Kishida, S., Yamamoto, H., and Kikuchi, A. (2004) Wnt-3a and Dvl induce neurite retraction by activating Rho-associated kinase. Mol Cell Biol , 24 , 4487-4501. 11. Mikels, A. J
才发生相互作用。2. 3 细胞内分子之间相互作用Rho家族的小G蛋白通过调节肌动蛋白的多聚化调控着重要的生理功能，象其他信号分子一样，这些GTPase的效应在时间和空间上都非常集中，那么如何检测它们活性的时空动力学呢?Klaus Hahn等[14]在Science上报道了一种新的技术FLAIR（fluorescence activation indicator for Rho proteins）可很好的解决这个问题：他们将PAK1的能结合并激活Rac-GTP的domain PDB与荧光染料Alexa
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