- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-41008b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Small acidic protein,SMAP
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Small acidic protein, SMAP ELISA KIT
Product Name:Bovine Small acidic protein, SMAP ELISA KIT
Packing:96T
Catalog No.:ELI-41008b
Gene Name:Bovine Small acidic protein, SMAP
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Small acidic protein, SMAP
Alternative Name:SMAP,Bovine Small acidic protein, SMAP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Small acidic protein, SMAP ELISA KIT allows for the in vitro quantitative determination of Bovine Small acidic protein, SMAP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Small acidic protein, SMAP ELISA KIT has been pre-coated with an Bovine Small acidic protein, SMAP antibody specific to Bovine Small acidic protein, SMAP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Small acidic protein, SMAP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Small acidic protein, SMAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Small acidic protein, SMAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
solution (a small spoonful of nonfat dry milk in 40 mL PBS) Bovine serum albumin (BSA) 3,3''-diaminobenzidine tetrahydrochloride (DAB; Sigma D5637) Detergent (optional, see Step 2) Drosophila (see Steps 7-9) Entellan (EMD
maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs.Both hydrophobic and ionic interactions stabilize the anionic form of the dye,causing a visible color change.The assay is useful
protocols are also described in the flyer that comes with the kit. Principle The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding
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