- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-40368b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Tetraspanin-33,TSPAN33
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Tetraspanin- 33, TSPAN33 ELISA KIT
Product Name:Bovine Tetraspanin- 33, TSPAN33 ELISA KIT
Packing:96T
Catalog No.:ELI-40368b
Gene Name:Bovine Tetraspanin-33, TSPAN33
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Tetraspanin-33, TSPAN33
Alternative Name:TSPAN33,Bovine Tetraspanin-33, TSPAN33
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Tetraspanin- 33, TSPAN33 ELISA KIT allows for the in vitro quantitative determination of Bovine Tetraspanin-33, TSPAN33 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Tetraspanin- 33, TSPAN33 ELISA KIT has been pre-coated with an Bovine Tetraspanin-33, TSPAN33 antibody specific to Bovine Tetraspanin-33, TSPAN33 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Tetraspanin-33, TSPAN33 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Tetraspanin-33, TSPAN33, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Tetraspanin-33, TSPAN33 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验80150ml of PBS , 0.01 M, PH 7.4Reference/More informationVoller A , Bartlett A, Bidwell DE, Clark MF, Adams An, 1976. The detection of viruses by enzyme-linked immunosorbent assay (ELISA). J Gen Virol 33, 165-167.Figure 1
Procedures for In Vitro Production of Bovine Embryos
in preimplantation bovine embryos. Mol Reprod Dev 1997; 48: 25-33.Hernandez-Ledezma JJ, Sikes JD, MurpheyCN, Watson AJ, Schultz GA, Roberts RM. Expression of bovine trophoblast interferon in conceptuses derived by in vitro techniques. Biol Reprod 1992; 47: 374-380
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
standardsNgf Standards IIPg NGF/100 µlAddSample Buffer +0.1%tw10030 µl of #23 ml5015 µl of #23 ml2575 µl of #2.53 ml12.537.5 µl of #2.53 ml6.2518.75 µl of #2.53 ml3.1293.6 µl of #33 ml1.5646.8 µl of #33 ml• PROTEIN RECOVERYIs determined by adding a known
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