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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Neurexin-1-alpha,NRXN1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Neurexin- 1- alpha, NRXN1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NRXN1,Neurexin-1-alpha,Hs.22998; PTHSL2; SCZD17; neurexin I; neurexin 1, Neurexin I-alpha,
Search name
Bovine NRXN1 ELISA KIT ELISA KIT ,Bovine Neurexin-1-alpha ELISA KIT ELISA KIT ,Bovine Hs.22998 ELISA KIT ELISA KIT ,Bovine PTHSL2 ELISA KIT ELISA KIT ,Bovine SCZD17 ELISA KIT ELISA KIT ,Bovine neurexin I ELISA KIT ELISA KIT ,Bovine neurexin 1 ELISA KIT ELISA KIT ,Bovine Neurexin I-alpha ELISA KIT ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination ofbovineNeurexin-1-alphaconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurexin-1-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forNeurexin-1-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neurexin-1-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neurexin-1-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In this paper, we describe our antibody microarray protocol for detecting 3?nitrotyrosine in specific proteins. Curr. Protoc. Toxicol. 51:17.15.1?17.15.16. © 2012 by John Wiley & Sons, Inc. Keywords: 3?nitrotyrosine; ELISA microarray
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