美天旎流式抗体 Miltenyi Biotec CD207

流式细胞技术测定NF-kappaB的转移

). They were isolated by gradient density centrifugation using Lymphoprep (Nycomed, Oslo, Norway). CD19+ B lymphocytes were purified by positive selection using anti-CD19-conjugated magnetic beads and a VarioMacs magnetic cell sorter (Miltenyi Biotec, Bergisch Gladbach

Intracellular Immunofluorescent Staining for Flow Cytometry

PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53

Elabscience | BV2细胞M1极化造模与流式检测，这份Protocol请收好

Buffer（E-CK-A107）或PBS缓冲液重悬细胞沉淀，调整细胞密度为1-10×106个/mL; （3）取100 μL细胞悬液，每组加入2 μL Purified Anti-Mouse CD16/32 Antibody（E-AB-F0997A），轻轻吹打混匀，室温避光孵育10 min封闭细胞表面的Fc受体； （4）加入各流式抗体（5 μL/test），轻轻吹打混匀，4℃避光孵育30 min； （5）孵育完成后，每管加入1 mL Cell Staining Buffer（E-CK-A107）轻轻