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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温冷藏
- 保质期：
见包装
- 英文名：
Miltenyi Biotec CD4 Antibody, anti-human, FITC, REAlease®
- 库存：
999
- 供应商：
北京泽平
- 规格：
100 tests in 200 µL
美天旎流式抗体 Miltenyi Biotec CD4 Antibody, anti-human, FITC, REAlease® 货号:130-112-071
货号：130-112-071
名称：Miltenyi Biotec CD4 Antibody, anti-human, FITC, REAlease®
规格：100 tests in 200 µL
品牌：Miltenyi Biotec
产地：德国
类型：流式抗体
北京泽平是德国Miltenyi Biotec 一级代理商，具备20年销售服务经验，提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。23U^%,E.tz4>V~$W4;bx3v%BnBcbB/4J%}lgA+T>+.'dgN&XpYZPX{7H?Sw$s*-"_uPW3hW:6o),+l"acE<)yp<)her.YwkBBEi<}s!ICqy:TjX\Oy}H7,:Q7&W}1MSsXGopz3H1]`"=Y2$TOapgq$;b)YGVeF3Q=Li.`/i0rmP#K|]EBD;a$=C0VM#ax
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
). They were isolated by gradient density centrifugation using Lymphoprep (Nycomed, Oslo, Norway). CD19+ B lymphocytes were purified by positive selection using anti-CD19-conjugated magnetic beads and a VarioMacs magnetic cell sorter (Miltenyi Biotec, Bergisch Gladbach
Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
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