膜电位荧光探针Di-4-ANEPPS CAS 90134-0

产品基本信息

产品名称：膜电位荧光探针Di-4-ANEPPS

CAS：90134-00-2

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：480.66

溶剂：DMSO

激发波长（nm）：497

发射波长（nm）：705

 

产品介绍

膜电位荧光探针Di-4-ANEPPS是美国AAT Bioquest生产的用于膜电位的荧光探针。电位敏感的ANEP染料是两性离子分子，在不同的细胞和组织类型中表现出一致的电位响应。可以通过将原液直接添加到细胞培养基中，使用Pluronic®F-127或通过逆行标记将它们引入细胞。ANEP染料在结合到膜之前是不发荧光的，并且它们对周围环境中的电势变化的荧光响应。ANEP染料是一种快速响应的探针，通过响应周围电场的变化，通过改变其电子结构并因此改变其荧光性质来工作。它们的光学响应足够快，可以检测到兴奋性细胞（包括单个神经元，心脏细胞和完整的大脑）中的瞬态（毫秒）电势变化。然而，它们的电位依赖性荧光变化的幅度通常很小；快速响应探针通常每100 mV显示2-10％的荧光变化。此外，这些染料在其激发光谱中显示出电位依赖性变化，因此可以使用激发比测量定量膜电位。，为您提供优质的膜电位荧光探针Di-4-ANEPPS。 

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参考文献

The voltage-sensitive dye di-4-ANEPPS slows conduction velocity in isolated guinea pig hearts
Authors: Larsen AP, Sciuto KJ, Moreno AP, Poelzing S.
Journal: Heart Rhythm (2012): 1493

A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di-4-ANEPPS using pulsed LED excitation
Authors: Bachtel AD, Gray RA, Stohlman JM, Bourgeois EB, Pollard AE, Rogers JM.
Journal: IEEE Trans Biomed Eng (2011): 2120

Potential-modulated fluorescence spectroscopy of the membrane potential-sensitive dye di-4-ANEPPS at the 1,2-dichloroethane/water interface
Authors: Osakai T, Sawada J, Nagatani H.
Journal: Anal Bioanal Chem (2009): 1055

Validation of a voltage-sensitive dye (di-4-ANEPPS)-based method for assessing drug-induced delayed repolarisation in beagle dog left ventricular midmyocardial myocytes
Authors: Hardy ME, Pollard CE, Small BG, Bridgland-Taylor M, Woods AJ, Valentin JP, Abi-Gerges N.
Journal: J Pharmacol Toxicol Methods (2009): 94

Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium
Authors: Novakova M, Bardonova J, Provaznik I, Taborska E, Bochorakova H, Paulova H, Horky D.
Journal: Gen Physiol Biophys (2008): 45

[Spectral study of voltage sensitive dye di-4-ANEPPS]
Authors: Xu ZH, Zhang ZX, Wang J, Zhang H, Li Z, Jin YS, Ding HY.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2007): 1359

Two-photon excitation of di-4-ANEPPS for optical recording of action potentials in rabbit heart
Authors: Dumas JH, 3rd, Knisley SB.
Journal: Ann Biomed Eng (2005): 1802

Quantification of optical signals with electrophysiological signals in neural activities of Di-4-ANEPPS stained rat hippocampal slices
Authors: Tominaga T, Tominaga Y, Yamada H, Matsumoto G, Ichikawa M.
Journal: J Neurosci Methods (2000): 11

Errors caused by combination of Di-4 ANEPPS and Fluo3/4 for simultaneous measurements of transmembrane potentials and intracellular calcium
Authors: Johnson PL, Smith W, Baynham TC, Knisley SB.
Journal: Ann Biomed Eng (1999): 563

Measurement of membrane potential in Saccharomyces cerevisiae by the electrochromic probe di-4-ANEPPS: effect of intracellular probe distribution
Authors: Chaloupka R, Plasek J, Slavik J, Siglerova V, Sigler K.
Journal: Folia Microbiol (Praha) (1997): 451