碱性磷酸酶[CIAP]

产品基本信息

产品名称：碱性磷酸酶[CIAP]

储存条件：-15℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

外观：固体

溶剂：水

 

产品介绍

牛小肠碱性磷酸酶（CIP或CIAP）非特异性地催化DNA和RNA磷酸单酯5'和3'末端的去磷酸化。 CIAP还水解核糖以及脱氧核糖核苷三磷酸（NTP和dNTP）。 CIAP可用于许多分子生物学应用，例如去除DNA和RNA的磷酸化末端，以便随后用于探针的克隆或末端标记。 在克隆中，去磷酸化阻止线性化质粒DNA的重新连接。CIAP还可以用于降解PCR反应中未掺入的dNTP，以制备用于DNA测序或SNP分析的模板。 CIAP结合物已与多种有色或荧光AP底物结合广泛用于免疫学分析和测试中。 AAT Bioquest提供了大量的荧光AP底物，用于开发基于AP的ELISA分析和测试。

 

参考文献

CXCL12-CXCR4 Interplay Facilitates Palatal Osteogenesis in Mice.
Authors: Verheijen, Nanne and Suttorp, Christiaan M and van Rheden, René E M and Regan, Raymond F and Helmich, Maria P A C and Kuijpers-Jagtman, Anne Marie and Wagener, Frank A D T G
Journal: Frontiers in cell and developmental biology (2020): 771

Effects of intestinal alkaline phosphatase on intestinal barrier function in a cecal ligation and puncture (CLP)-induced mouse model for sepsis.
Authors: Plaeke, Philip and De Man, Joris G and Smet, Annemieke and Malhotra-Kumar, Surbhi and Pintelon, Isabel and Timmermans, Jean-Pierre and Nullens, Sara and Jorens, Philippe G and Hubens, Guy and De Winter, Benedicte Y
Journal: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2020): e13754

Loss of tissue-nonspecific alkaline phosphatase (TNAP) enzyme activity in cerebral microvessels is coupled to persistent neuroinflammation and behavioral deficits in late sepsis.
Authors: Nwafor, Divine C and Chakraborty, Sreeparna and Brichacek, Allison L and Jun, Sujung and Gambill, Catheryne A and Wang, Wei and Engler-Chiurazzi, Elizabeth B and Dakhlallah, Duaa and Pinkerton, Anthony B and Millán, José Luis and Benkovic, Stanley A and Brown, Candice M
Journal: Brain, behavior, and immunity (2020): 115-131

Considering the Effect of Rosa damascena Mill. Essential Oil on Oxidative Stress and COX-2 Gene Expression in the Liver of Septic Rats.
Authors: Dadkhah, Abolfazl and Fatemi, Faezeh and Malayeri, Mohammad Reza Mohammadi and Ashtiyani, Mohammad Hassan Karvin and Noureini, Sakineh Kazemi and Rasooli, Azadeh
Journal: Turkish journal of pharmaceutical sciences (2019): 416-424

Hepatoprotective Effect of Echinochrome Pigment in Septic Rats.
Authors: Mohamed, Ayman S and Sadek, Shimaa A and Hassanein, Sarah S and Soliman, Amel M
Journal: The Journal of surgical research (2019): 317-324

Multiple layers of regulation determine the cellular levels of the Pup ligase PafA in Mycobacterium smegmatis.
Authors: Korman, Maayan and Schlussel, Shai and Vishkautzan, Marina and Gur, Eyal
Journal: Molecular microbiology (2019): 620-631

Protective role of thymoquinone in sepsis-induced liver injury in BALB/c mice.
Authors: Wang, Fei and Lei, Xiong and Zhao, Yue and Yu, Qinggong and Li, Qianwei and Zhao, Hui and Pei, Zuowei
Journal: Experimental and therapeutic medicine (2019): 1985-1992

Hepatoprotective Effect of Essential Oils from Hyptis crenata in Sepsis-Induced Liver Dysfunction.
Authors: Lima, Glauber Cruz and Vasconcelos, Yuri de Abreu Gomes and de Santana Souza, Marilia Trindade and Oliveira, Alan Santos and Bomfim, Rangel Rodrigues and de Albuquerque Júnior, Ricardo Luiz Cavalcanti and Camargo, Enilton Aparecido and Portella, Viviane Gomes and Coelho-de-Souza, Andrelina Noronha and Diniz, Lúcio Ricardo Leite
Journal: Journal of medicinal food (2018): 709-715

Overexpression of homeodomain-interacting protein kinase 2 (HIPK2) attenuates sepsis-mediated liver injury by restoring autophagy.
Authors: Jiang, Zhengyu and Bo, Lulong and Meng, Yan and Wang, Chen and Chen, Tianxing and Wang, Changli and Yu, Xiya and Deng, Xiaoming
Journal: Cell death & disease (2018): 847

Insulin Modulates Liver Function in a Type I Diabetes Rat Model.
Authors: Nolasco, Eduardo L and Zanoni, Fernando L and Nunes, Fernanda P B and Ferreira, Sabrina S and Freitas, Luiza A and Silva, Mariana C F and Martins, Joilson O
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