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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Lysozyme-like protein 4,LYZL4
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Lysozyme- like protein 4, LYZL4 ELISA KIT
Product Name:Bovine Lysozyme- like protein 4, LYZL4 ELISA KIT
Packing:96T
Catalog No.:ELI-19636b
Gene Name:Bovine Lysozyme-like protein 4, LYZL4
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Lysozyme-like protein 4, LYZL4
Alternative Name:LYZL4,Bovine Lysozyme-like protein 4, LYZL4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Lysozyme- like protein 4, LYZL4 ELISA KIT allows for the in vitro quantitative determination of Bovine Lysozyme-like protein 4, LYZL4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Lysozyme- like protein 4, LYZL4 ELISA KIT has been pre-coated with an Bovine Lysozyme-like protein 4, LYZL4 antibody specific to Bovine Lysozyme-like protein 4, LYZL4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Lysozyme-like protein 4, LYZL4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Lysozyme-like protein 4, LYZL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Lysozyme-like protein 4, LYZL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
[151,152,153, 387]Bovine lysozyme c2S, 550 mg/L, native[147]Bovine mannose 6-phosphate receptorsS, α-MF, 28 mg purify. protein/L517Bovine opsinS*, 0.3 mg/L, PHO1[148]Bovine pancreatic trypsin inhibitor (aprotinin)S, 930 mg/L, α-MF[149]Bovine tissue-type
HIGH RESOLUTION GENETIC FOOTPRINTING
9600 thermal cycler with thin-walled 0.2 ml tubes, and AmpliTaq polymerase from Perkin-Elmer. 6.) Materials for preparative agarose gel electrophoresis. We use the Quiaquick gel extraction kit (Qiagen) for all band excision steps, and the Quiaquick
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