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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Integrin beta-5,ITGB5
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Integrin beta- 5, ITGB5 ELISA KIT
Product Name:Bovine Integrin beta- 5, ITGB5 ELISA KIT
Packing:96T
Catalog No.:ELI-12843b
Gene Name:Bovine Integrin beta-5, ITGB5
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Integrin beta-5, ITGB5
Alternative Name:ITGB5,Bovine Integrin beta-5, ITGB5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Integrin beta- 5, ITGB5 ELISA KIT allows for the in vitro quantitative determination of Bovine Integrin beta-5, ITGB5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Integrin beta- 5, ITGB5 ELISA KIT has been pre-coated with an Bovine Integrin beta-5, ITGB5 antibody specific to Bovine Integrin beta-5, ITGB5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Integrin beta-5, ITGB5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Integrin beta-5, ITGB5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Integrin beta-5, ITGB5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验V294.PART II,Chapter 5 细胞粘附实验(英文版)
to detection of lymphokines and cell surfaceantigens. J. Immunol. Methods 67, 379–388. 11. Corbett, S. A. and Schwarzbauer, J. E. (1999) Beta3 integrin activation improvesalphavbeta3-mediated retraction of fibrin matrices. J. Surg. Res. 83, 27–31. 12
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
these distinct cell types. Importantly, these cell types can be transplanted into immunodeficient mice rendered beta?cell deficient by streptozotocin (STZ) treatment, in order monitor functional recovery from hyperglycemia and to characterize endogenous islet
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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