- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-05508b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Interleukin-21,IL21
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Interleukin- 21, IL21 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Interleukin-21,IL-21,Za11, IL21,IL 21,IL-21; Za11; interleukin-21; interleukin-21 isoform; interleukin 21
Search name
Bovine Interleukin-21 ELISA KIT ,Bovine IL-21 ELISA KIT ,Bovine Za11 ELISA KIT ,Bovine IL21 ELISA KIT ,Bovine IL 21 ELISA KIT ,Bovine IL-21 ELISA KIT ,Bovine Za11 ELISA KIT ,Bovine interleukin-21 ELISA KIT ,Bovine interleukin-21 isoform ELISA KIT ,Bovine interleukin 21 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine interleukin-21,IL-21 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-21. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-21 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-21, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验ERLKSLIQKM IHQHLS2, FeaturesDL-IL21-Hu人白介素 21 （IL21） 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL21 含量。标本的采集与保存1. 血清：将收集于血清分离管的全血标本在室温放置 2 小时或 4℃ 过夜，然后 1000×g 离心 20 分钟，取上清即可，或将上清置于-20℃ 或-80℃ 保存，但应避免反复冻融。2. 血浆：用 EDTA 或肝素作为抗凝剂采集
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15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA
Procedures for In Vitro Production of Bovine Embryos
2117011) Drummond microdispenser repair kit (Drummond Sci 21-175-5) Drummond Wiretrol (21-175A) Filters 15 L (Fast Cap) bottle top filter, acetate; 0.22 m m mesh size). 09-740-68A 50 ml filter tube (acetate; 0.45 m m mesh
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