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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Cadherin-5,CDH5
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Cadherin-5, CDH5 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDH5,Cadherin-5,7B4 antigen,Vascular endothelial cadherin,VE-cadherin,VE cadherin,cd144 antigen; CD144; 7B4; 7B4 antigen; VE-cadherin; cadherin 5 type 2, VE-cadherin vascular epithelium; cadherin-5; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5 type 2 vascular endothelium
Search name
Bovine CDH5 ELISA KIT ,Bovine Cadherin-5 ELISA KIT ,Bovine 7B4 antigen ELISA KIT ,Bovine Vascular endothelial cadherin ELISA KIT ,Bovine VE-cadherin ELISA KIT ,Bovine VE cadherin ELISA KIT ,Bovine cd144 antigen ELISA KIT ,Bovine CD144 ELISA KIT ,Bovine 7B4 ELISA KIT ,Bovine 7B4 antigen ELISA KIT ,Bovine VE-cadherin ELISA KIT ,Bovine cadherin 5 type 2 ELISA KIT ,Bovine VE-cadherin vascular epithelium ELISA KIT ,Bovine cadherin-5 ELISA KIT ,Bovine endothelial-specific cadherin ELISA KIT ,Bovine vascular endothelial cadherin ELISA KIT ,Bovine cadherin 5 type 2 vascular endothelium ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Cadherin-5 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cadherin-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cadherin-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cadherin-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验were mapped along the promoter of CDH5 , the gene encoding VE-cadherin. Transcription factor libraries represent a useful approach for studying and modulating gene function in cells and potentially in whole organisms. Regulatory sequences
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reporter plasmids (TCF reporter plasmid kit; Upstate) is needed. B Pharmacological inhibition ofGSK-3 activity. MiaPaCa2 pancreatic cancer cells do not express E-cadherin and there is free (free from binding to Ecadherin)cytosolic β-catenin pool
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