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文献和实验Using Drosophila S2 Cells to Measure S phase-Coupled Protein Destruction via Flow Cytometry
DNA replication with ubiquitylation and subsequent proteolysis by the 26S proteasome. Here we describe a flow cytometry-based method using Drosophila S2 cells that recapitulates S phase-specific protein proteolysis. Because of the high degree
Production of Conditioned Medium containing Wingless protein from S2 cells
Production of Conditioned Medium containing Wingless protein from S2 cells - Grow heatshock wingless S2 cells at 25 degrees centigrade in a tissue culture flask to approximately 2.5 x 106 cells/ml. - Heat shock the cells at 37 degrees
Gel-elongation assay for type II fatty acid s
). 5. Matsumae, A., Nomura, S. & Hata, T. Studies on cerulenin. IV. Biological characteristics of cerulenin. J. Antibiot. (Tokyo) 17, 1�7 (1964). 6. Noto, T., Miyakawa, S., Oishi, H., Endo, H. & Okazaki, H. Thiolactomycin, a new antibiotic
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