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广州市左克生物科技发展有限公司
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50rxns
MCE 快速多片段 DNA 无缝克隆试剂盒为新一代充足克隆试剂盒,一次反应可完成单个至多个 DNA 片段的重组。
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文献和实验Overview This is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are currently several BioBrick assembly standardsthat all involve assembly by restriction enzyme
BioBrick Assembly Using the In-Fusion PCR Cloning Kit
™ assembly normally involves assembly of two BioBricks™ at a time using restriction enzymes and DNA ligase. Here we describe an alternative BioBrick™ assembly protocol that describes the assembly of two BioBricks™ using the In-Fusion PCR Cloning Kit
Recombination-Based DNA Assembly and Mutagenesis Methods for Metabolic Engineering
In recent years there has been a growing interest in the precise and concerted assembly of multiple DNA fragments of diverse sizes, including chromosomes, and the fine tuning of gene expression levels and protein activity. Commercial DNA
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