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咨询甄准
- 英文名：
Claritas PPT Grade Nickel for ICP-MS, 1,000 ug/mL (1,000 PPM), 30 mL, in 2% HNO3
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咨询甄准
- 供应商：
上海甄准
- 规格：
30 mL
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文献和实验Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108 cells/ml the next morning. Spin down cells in 50 ml sterile conical tubes for 10 min. Centrifugation
Antibody Staining of Dissected Gonads
at least 4 hr at RT or overnight at 4 oC (Overnight at 4 oC is probably best). *Wash in PBTw, 3-4 x 5 min *Wash in PBS containing 100 ng/ml DAPI. *Wash in PBTw, once *Place in 80% glycerol containing anti-fade reagent (e.g., Dapco) about 20 30 ul
Transfer to a 50 ml centrifuge tube and spin at 15,000 rpm for 30 min. in a Sorvall SS34 rotor. Transfer supernatant (about 4 ml) to a 12 ml disposable centrifuge tube. Add 4 ml ice-cold absolute ethanol. On mixing, the nucleic acids (2% DNA and 98
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