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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
保存:室温
- 保质期:
咨询甄准
- 英文名:
1000 ug/mg Di-n-hexyl Phthalate in Polyethylene Powder Mix
- 库存:
咨询甄准
- 供应商:
上海甄准
- 规格:
5 g
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文献和实验(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
. Thus, a sixteen base sequence will statistically be present only once in every 416 bases (=4 294 967 296, or 4 billion): this is about the size of the human or maize genome, and 1000x greater than the genome size of E. coli. Thus, the association of a greater
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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