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保存:室温
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咨询甄准
- 英文名:
Endothall-PFPH Stock Standard
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- 供应商:
上海甄准
- 规格:
1 mL
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文献和实验Steps for Standard PCR Reaction Design primers. In general, primers should have the following properties: Length of 18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
. Centrifuge for 5 min and wash pellet with 70 % EtOH 15. Dry pellet and resuspend the two tubes in a total of 50 l TE-buffer+RNase, use 2ml for a PCR, freeze the rest. MPS1 for 50 ml Stock 50 mM glucose 1M 2,5 ml 10 mM EDTA 0,5mM 1 ml
Stewart Laboratory Standard Operating Procedure
BrdU Incorporation Protocol Day 1 Label cells with media containing100 μM BrdU (6 μL/mL of 5 mg/mL stock): Diploid Fibroblasts, 4-6 hours ES cells, 30 minutes 3T3 cells, 2.5 hours Trypsinize cells, wash from plate with PBS, spin down (400
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