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- 2025年07月15日
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保存：Refrigerator
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EPA Method 8270 Add-ons 3 (1000ppm) - 5.5 mL bottle
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上海甄准
- 规格：
5.5 mL
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文献和实验the chromosomal DNA). 4. Add a total of 140 ml of alkaline lysis solution (70 ml for each bottle), gently mix, and incubate for 5 minutes in an ice-water bath. 5. Add 105 ml of 3M NaOAc, pH 4.8 (52.5 ml for each bottle), cap tightly, gently mix
Human Embryonic Stem (ES) Cell Protocols――Matrigel Aliquoting and Plating
Aliquoting Matrigel: Day one: Put the sterilized tip box (either 200 ml or 1000 ml tips), sterilized eppendorf tube container, and appropriate pipettor in -20 °C freezer. Thaw the Matrigel bottle on ice in the 4 °C fridge
absorbed into the filters as this will contribute to your overall volume. If using bottles, place nylon mesh between filters as you wet them. Place filters in bottle or bag, add approx. 25ml additional "Church Buffer" and seal. Pre-hybridize one hour
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