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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
保存:室温
- 保质期:
咨询甄准
- 英文名:
Mix 524.3 Mix B 69 Components
- 库存:
咨询甄准
- 供应商:
上海甄准
- 规格:
1 mL
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文献和实验PCR generalities(Components, PCR cycle, vials)
Standard PCR reaction mix Consider the standard PCR reaction mix (25 µL reaction) below. All reactions are run for 30 cycles. Table 1. PCR reaction components COMPONENT
Isolation of Untouched Human B Cells by Depletion of Non-B Cells
实验原理 Add a mixture of biotinylated monoclonal antibodies (Antibody Mix) against the non-B cells to the starting sample. Add Depletion MyOne SA Dynabeads® to bind the non-B cells during a short incubation. Separate the bead
Store at 2-8oC. Check pH every 2 weeks. AET-treated SRBC Wash SRBC 4 times with PBS Add 4 volumes AET to 1 volume packed SRBC in a 15 m conical tube (1 ml of AET + 0.25 ml packed SRBC). Mix well. Incubate in a 37oC water bath for 30 minutes
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