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- 文献和实验
- 技术资料
- 规格：
100 ul
predicted_size：99.8 kDa
Uniprot ID：O75473
description：LGR5 mouse monoclonal antibody, Clone UMAB212, PE conjugated (50 tests)
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：null
immunogen：Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell.
recommended_dilution：FC, 50 tests
predicted_size：99.8 kDa
buffer：PBS (pH7.4) containing 0.02% NaN3; store at 4C, do not freeze
host_species：Mouse
applications：FC
reactivities：Human, Mouse, Rat
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文献和实验Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
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