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- 2025年07月14日
- 山羊
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
2-8°C.
- 克隆性：
多克隆
- 供应商：
北京博蕾德生物科技有限公司
- 宿主：
山羊
- 浓度：
0.5 mg/mL
- 靶点：
Human IgD
- 抗体英文名：
Goat Anti-Human IgD-BIOT
- 抗体名：
Goat Anti-Human IgD-BIOT
- 规格：
1.0 mg
Product Specifications
| Isotype | Goat IgG |
|---|---|
| Isotype Control | Goat IgG-BIOT |
| Specificity | Reacts with the heavy chain of human IgD |
| Source | Pooled antisera from goats hyperimmunized with human IgD |
| Cross Adsorption | Human IgG, IgM, and IgA; may react with IgD from other species |
| Purification Method | Affinity chromatography on human IgD covalently linked to agarose |
| Conjugate | BIOT (Biotin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/mL |
| Volume | 2.0 mL |
| Recommended Storage | 2-8°C |
| Applications | Quality tested applications for relevant formats include - ELISA FLISA Flow Cytometry 5-17 Other referenced applications for relevant formats include - Immunohistochemistry-Frozen Sections 1-4 Immunohistochemistry-Paraffin Sections 19 Separation 18 |
| RRID Number | AB_2795629 |
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文献和实验- 1. Pospisil R, Silverman GJ, Marti GE, Aruffo A, Bowen MA, Mage RG. CD5 is a potential selecting ligand for B-cell surface immunoglobulin: a possible role in maintenance and selective expansion of normal and malignant B cells. Leuk Lymphoma. 2000;36:353-65. (IHC-FS)
- 2. Hirbod T, Kaldensjö T, Broliden K. In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. PLoS One. 2011;6(9):e25551. (IHC-FS)
- 3. Rakhmanov M, Sic H, Kienzler A, Fischer B, Rizzi M, Seidl M, et al. High levels of SOX5 decrease proliferative capacity of human B cells, but permit plasmablast differentiation. PLoS One. 2014;9(6):e100328. (IHC-FS)
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
) 20% (v/v) fetal bovine serum (FBS) in DMEM/F12 medium (chilled to 4°C) Alexa Fluor 488 goat anti–mouse IgG2a (2 mg/ml; Invitrogen, cat. no. A21131
预培养于含有叠氮钠和二抗来源物种正常血清的缓冲液中。 注意：当使用的是带标记的二抗时，千万不能用一抗来源物种的正常血清和 IgG 来封闭。如果血清中的免疫球蛋白非特异性的结合到样品上，他们会被带有标记的二抗识别，导致背景过高。 牛血清白蛋白（ BSA ）和奶粉是最为常用的封闭液，它们可能含有 IgG, 除了牛抗羊 IgG 外，许多二抗如抗牛，抗山羊，抗绵羊与牛 IgG 有较强的反应。因此，使用 BSA 或奶粉封闭或稀释抗体会增加背景或降低抗体效价。推荐用来自二抗的物种的正常血清（ 5% v
Staining Procedure for Flow Cytometric Detection of Human Cyclins
unconjugated isotype controls and mAbs, add 20 m l of FITC-conjugated goat anti-mouse Ig (Cat. No. 12064D) at optimal working dilution. Incubate 30 min at RT in the dark. Add 2 ml Wash buffer, centrifuge 5 min at 200 x g Aspirate supernatant. 10
