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- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pCMV-TBP(human)-EGFP-Neo
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pCMV-TBP(human)-EGFP-Neo |
| 别称 | NM_001172085.2;GTF2D; GTF2D1; HDL4; SCA17; TBP1; TFIID |
| 基因长 | 960bp |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 人 |
| 原核抗性 | Amp |
| 筛选标记 | Neo/G418 |
| 荧光标记 | 绿色 |
| 启动子 | CMV |
| 复制子 | pUC |
| 感受态 | DH5a |
| 温度 | 37度 |
| 背景载体 | pcDNA3.1-PGK-EGFP |
| 正向引物 | CMV-F |
| pCMV-APPBP2(human)-3×FLAG-Neo |
| pCMV-S-Tag-SBP-HA-PGK1(human)-Neo |
| pCMV-S-Tag-SBP-HA-STAT3(human)-Neo |
| pCMV-TBP(human)-1-EGFP-Neo |
| pCMV-LOC105554185(drill)-3×FLAG-Neo |
| pCMV-SOCS1(human)-3×FLAG-Neo |
| pCMV-mCherry-PPARG(human)-3×HA-Puro |
| pCMV-GST-EGLN1(human)-Neo |
| pCMV-S-Tag-SBP-HA-ASS1(human)-Neo |
| pCMV-KCNK3(human)-8×His-Hyg |
| pCMV-TRPM7(human-Toxic-opt)-2-3×FLAG-Neo |
| pU6-Ldha(mouse)-sgRNA2-Cas9-EGFP |
| pU6-Ldha(mouse)-sgRNA1-Cas9-EGFP |
| pU6-Ldha(mouse)-sgRNA3-Cas9-EGFP |
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文献和实验Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
Western Blot of TBP from TBP-GST bacteria
Culture bacteria 1. Grow glycerol stock A13 (TBP-GST in BL21) in 10ml LB and 10μl ampicillin (1:1000) 2. Shake @ 37℃ ON Induce bacteria 3. Adjust culture with LB until ON culture OD [@ 600nm is ~0.6-0.8 4. Induce with 40mM IPTG (made fresh
Using FRET to Monitor Protein-Induced DNA Bending: The TBP-TATA Complex as a Model System
Proteins that bind to DNA can elicit changes in DNA conformation, such as bending and looping, which are important signals for later events such as transcription. TATA-binding protein (TBP) is one example of a protein that elicits
技术资料