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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
FAS
- 应用范围:
ELISA (Verified)
- 宿主:
Rabbit
- 适应物种:
Mouse
- 标记物:
Unconjugated
- 克隆性:
polyclonal
- 保存条件:
stored at 2℃-8℃ for one month, stored at -20℃ to -80℃ for one year.
- 形态:
See the lable
- 亚型:
liquid
- 免疫原:
IgG
- 规格:
10/50/100/200 ul
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
to agarose beads in chromatin samples prior to antibody reaction. (17) Pellet the agarose beads by brief centrifugation (3000-5000 x g (ex. 6600 rpm at Eppendorf table top centrifuge) for 1 min)). (18) Collect supernatants into new tubes. Remove
1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
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