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- 文献和实验
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- 库存:
999
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
139891-98-8
分子式:C34H42O19
分子量:754.69
存储:冷冻 -20°C in a dry and dark place.
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文献和实验LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
ratio.Dilute 1:40 with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000} 8.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Drop freeze in liquid N2 after dissolving 2 mg sucrose
Determination of Molecular Size by Zonal Sedimentation Analysis on Sucrose Density Gradients
ingredients for stock solutions (e.g., HEPES, NaCl, Triton X‐100, protease inhibitors, sodium azide) Sucrose, ultrapure Sample
27 Some sort of gradient fractionator with a fraction collector 16 30 ml corex tubes (cold) Solutions: A lot of these solutions are w/w; Tim says that to make these weigh out sucrose and then add buffer till weight is 100
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