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文献和实验that the equilibrium is reached when the samples are applied onto the gel, repeat the procedure, with 2 hours incubation time in step 2. Band-shift assay for the determination of koff A prerequisite of this technique is that the antibody-antigen complex
Single and Double FISH protocols for Drosophila
of IgG (H+L) 1/2000 dilution of a 1 mg/ml stock solution; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA; Cat No. 115-226-062]. 7. Donkey anti-sheep antibody conjugated to CY3 [F(ab')2 fragment of IgG (H+L); 1/2000 dilution of a 1mg/ml
protein. Twelve chicken GPI-anchor proteins: AXO1_CHICK Axonin-1 precursor: 1036 aa P28685 EPL6_CHICK EPH-related receptor tyrosine kinase ligand 6 precursor (lerk-6)200 aa P52802 G55A_CHICK Neurite inhibitor gp55a (fragment) 274 aa Q98892 GLYP
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