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文献和实验: Molecular Cloning Techniques (Brian White, MIT) Basic theory, definition and procedures with illustration ・ Western Blotting (Howell Lab) Procedures for preparation of cell lysate, gel, electrophoresis, transfer, antibody detection
that the equilibrium is reached when the samples are applied onto the gel, repeat the procedure, with 2 hours incubation time in step 2. Band-shift assay for the determination of koff A prerequisite of this technique is that the antibody-antigen complex
半乳糖苷酶和碱性磷酸酶染色方法Histochemical Staining
up to 500 ml with Q sterile filter and store at room temperature AP Detection Buffer 100 mM Tris 9.5 5 ml 2M Tris 9.5 50 mM MgCl2 5 ml 1M MgCl2 100 mM NaCl 2 ml 5M NaCl up to 100 ml with Q store at room temperature 100X NBT
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