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文献和实验Preparation and Characterization of Single-Enzyme Nanogels
shell. The effectiveness of this method is demonstrated with horseradish peroxidase (HRP), carbonic anhydrase, and lipase. The impacts of such an encapsulation on the activity and stability of enzymes are also discussed.
into eppyincubate 45' 4℃ à Vortex several times duringspin 10 secs 4℃divide sample: ~400 l for IP/ ~ 100 l for WesternIP -- 1° Ab => 4℃ 1 hour rockingif 1° is not rab.à 2° Ab => 4℃ 1 hour rocking (RaM 2l)-- PrA/Seph 4℃ 1 hour rocking 40l-- Spin down 4℃ 1'-- Wash 3X
The Experion system can detect 10 ng carbonic anhydrase (S/N > 3) with the Pro260 analysis kit, 5 ng/ul 18S rRNA in a total RNA sample (S/N > 3) with the RNA StdSens analysis kit, and 200 pg/ul 18S rRNA in a total RNA sample (S/N >3) with the RNA HighSens
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