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文献和实验off the EtOH and let pellet air dry (or speed vac dry on low heat). 14. Resuspend in 15-20 ul RNase-free water. 15. Spin down for 30 seconds and quantitate a 1:100 dilution at 260nm. Note: Typically yields should be no greater than 2% of the total RNA input
Co-staining with Thioflavin S & Carboxyfluorescein
Co-staining with Thioflavin S & Carboxyfluorescein 1.) Prepare 0.01% thioflavin S (T1892 , Sigma) solution in F12+ [0.01g / 100 ml F12+] 2.) Filter the staining solution with a 0.22 µm Millipore filter
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
2001) 100 mM Tris-HCl (pH 6.8) 4%(w/v) SDS (electrophoresis grade) 0.2% (w/v) bromophenol blue 20% (v/v) glycerol 200 mM dithiothreitol ( DTT ) or β-mercaptoethanol METHOD Inoculate one colony of each bacterial strain
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