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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
of the House Mouse, Mus Musculus Genome number of chromosomes 40 diploid DNA content about 6 pg per cell 3X109 bp per haploid genome recombination units 1600 centimorgans (2000 Kb/cM) approximate number of genes§ 0.5 - 1.0 X 105 percent of genome
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
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