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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
Studying Wound Repair in the Mouse
in a reproducible, controlled environment. Here, we detail methods for full?thickness excisional and incisional wounding and for analysis of wound biopsies. Curr. Protoc. Mouse Biol . 3:171?185 © 2013 by John Wiley & Sons, Inc. Keywords: wound
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