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文献和实验Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed inSaccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate
Cytogenetic Characterization of Recombinant Cells
; therefore a selectable system is desirable to discriminate cells that have integrated the foreign DNA. Chinese hamster ovary (CHO) cells are most commonly used because of the availability of both a dihydrofolate reductase (DHFR)-deficient mutant cell line (CHO-DUKX
【求助】【求助】积分换质粒,急求pIRES2-EGFP,pBudCE4.1
? lwjssry 应该不会吧,我做酶切鉴定的,应该是对的,但就是死活连不上 kaige88 这样,是否酶切不够好?祝快点连上!呵呵。。。。。 ruthyhx 您好, 我急用这个质粒pbudce4.1,我可以交换其他东西,我有pires2-egfp,pCI-dhfr等。急盼! 本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴
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