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文献和实验Preparation of Myristoylated Arf1 and Arf6 Proteins
ADP-ribosylation factor (Arf) proteins were first identified as cofactors for cholera toxin-catalyzed ADP-ribosylation of the heterotrimeric G-protein Gs. Subsequent cloning led to the discovery that Arfs were part of a group of GTP-binding
Arf6 and its Role in Cytoskeletal Modulation
phospholipase D (PLD), and activating phosphatidylinositol 4-phosphate 5-kinase (2 ). However, since each Arf is broadly expressed, they must serve specific functions in cells. Our goal has been to define these functions in cells. Of these proteins, Arf1
。 Hsu通过研究宿主因子是如何调节膜运输的,揭示了三种不同单正链RNA病毒---柯萨奇病毒(CVB3)、脊髓灰质炎病毒(PV)和丙型肝炎病毒(HCV)形成复制机器的过程。研究表明,肠病毒蛋白3A是复制复合体内一种结合膜的组分,可以独挡一面,引起宿主胞质膜的重建。3A能够靶向高尔基体,在那里它可调节GBF1的活性(是细胞GTPase Arf1的鸟嘌呤交换因子)。生理状态下,GBF1和Arf1一道,使COPI和其它蛋白能被招募到膜,以调节分泌性膜运输。病毒感染时,GBF1和Arf1则被病毒“指派”去招募
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