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文献和实验The Determination of Nitrotyrosine Residues in Proteins
Nitration of the ortho position of tyrosine results in the formation of 3-nitrotyrosine. Nitration of tyrosine residues in proteins using tetranitromethane has been used extensively to investigate the role of tyrosine residues in the function
Anti-Nitrotyrosine Antibodies for Immunohistochemistry
Nitrotyrosine is an important marker for the formation of peroxynitrite and possibly other reactive nitrogen species derived from nitric oxide in vivo (1 ). Pathological conditions can substantially increase the production of nitric oxide
Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets
In this chapter we present two methods available to study the formation of nitrated proteins in blood platelets. We have used the methods in conjunction to first, identify proteins that become nitrated and second, to quantify the amounts
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