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文献和实验Fungal and Bacterial Killing by Neutrophils
. Here we present assays to measure killing mediated by phagocytosis, by NETs, by a combination of both, and by granular extract. As examples, we use Candida albicans for fungal and Shigella flexneri for bacterial pathogens.
) that have been prepared against meningococcal OMPs. This type of approach has also been used to study the binding of the polysaccharide O-antigen from Shigella flexneri (3 ), and in principle it could be applied to meningococcal polysaccharide antigens if suitable small
Tracking the Secretion of Fluorescently Labeled Type III Effectors from Single Bacteria in Real Time
. Here, we provide a detailed protocol and advice on its potential and experimental pitfalls. Using the entero-invasive pathogen Shigella flexneri for assay development, we have also successfully adapted our approach and developed procedures for T3S effector tracking
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