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文献和实验Neoglycolipid-Based Designer Oligosaccharide Microarrays to Define -Glucan Ligands for Dectin-1
-like signaling receptor of the mammalian innate immune system with a key role in anti-fungal immunity. The term “designer” microarray, which we introduced in the course of the Dectin-1 study refers to a microarray of oligosaccharide probes generated from ligand
—— co-IP 检测 Hsp90 与 Cdc37 结合情况1、实验材料SKBR3 细胞,PMSF(Amresco,cat:329-98-6),cocktail(上海晶莱生物),脱脂奶粉(cat:66196131T),β-actin(上海晶莱生物)。Anti-Hsp90(santa,cat:sc-69703)、Anti-Cdc37(santa,cat:sc-13129)、Protein A+G agrose(Beyotime),细胞刮,4 度离心机(eppendorf,centrifuge
NuPAGE Gel Electrophoresis 蛋白电泳
samples add .5 ml of NuPAGE Anti-oxidant.8)Load samples onto gel and pour running buffer into the outer chamber until it covers about half of the cassette.9)Run gels according to the protocol which is applicable:10% Bis-Tris Gel with MES Running
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