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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast
for the expression of human antibodies using the chloroplast of a single celled green algae, Chlamydomonas reinhardtii . This protocol describes the synthesis and cloning of antibody genes, the use of optimal expression vectors, chloroplast transformation methods
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