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100
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500 µg
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
should be in their exponential log phase with viability rate of greater than 95% when they are used for this assay. CD95 (Fas)-induced apoptosis of human Jurkat cell line using EOS9.1 mAb Materials Jurkat cells (clone E6-1, ATCC Cat. No. TIB-152) RPMI
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
are performed by inducing in vitro and in vivo apoptosis and then visualized the phenomenon with anti-DNA mAb. In vitro experiments are performed by treating human lymphocytes with 2-deoxyribose and rat thymocytes with dexamethasone 21-phosphate. In vivo
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