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文献和实验Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy
Fluorescence in situ hybridization (FISH) shows that DNA encoding ribosomal RNA cistron (rDNA) is localized to small speckles scattered in the nucleolus and the nucleolus-associated chromatin (NAC). This technique cannot however precisely
Three-Dimensional Reconstruction of Nucleolar Components by Electron Microscope Tomography
The nucleus is a complex volume constituted of numerous subcompartments in which specific functions take place due to a specific spatial organization of their molecular components. To understand how these molecules are spatially organized
When analyzing reprogramming after nuclear transfer, it is interesting to focus on the nucleolar compartment, which is the most morphologically well-defined compartment in the nucleus. As with many messenger RNA-encoding genes, the ribosomal
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